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Cusabio csb el026133hu
Csb El026133hu, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/csb+el026133hu/Human+Protein+Wnt-2(WNT2)+ELISA+kit/pmc08669316-97-12-9
Average 92 stars, based on 4 article reviews

csb el026133hu - by Bioz Stars, 2026-10

92/100 stars

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Enzyme-linked Immunosorbent Assay:

Article Title: Elevated Wnt2 and Wnt4 activate NF-κB signaling to promote cardiac fibrosis by cooperation of Fzd4/2 and LRP6 following myocardial infarction
Article Snippet: .. Human Wnt2 and Wnt4 ELISA kits were purchased from CUSABIO company (Cat#: CSB-EL026133HU, CSB-EL026137HU, Wuhan, China) and used following the manufacturer's instructions. .. C57BL/6 male mice (8–10 weeks, Charles River Laboratories) were used to build MI model.

Article Title: Baseline Hedgehog Pathway Activation and Increase of Plasma Wnt1 Protein Are Associated with Resistance to Immune Checkpoint Inhibitors in Advanced Non-Small-Cell Lung Cancer
Article Snippet: .. We used commercially available ELISA kits (CSB-EL026128HU (Wnt1), CSB-EL026133HU (Wnt2), CSB-EL026135HU (Wnt3), Cusabio (Houston, TX, USA); ab100639 (Shh), Abcam, Cambridge, UK), according to the manufacturer’s instructions. .. Optical densities were determined with spectrophotometer (Bio-Rad PR3100) measuring optical density at 450 nm.

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Expression of Wnt2 in normal tissue and non-small cell lung cancers. (A) expression of Wnt2 in 8 representative pairs of NSCLC lung cancer tissues (T) and adjacent normal lung (N) tissues detected by RT-PCR (top) and Western blot (bottom). The results were processed with ImageJ (B). (C) immunohistochemical staining of Wnt2 protein in 5 representative pairs of histologic types of NSCLC (ADC, BAC, ASC, SCC and LCC) and adjacent normal lung tissues. (D) examples are shown for absent, low, median and high Wnt2 expression in NSCLC cancer tissues. (E) distribution of Wnt2 immunohistochemical results in 264 NSCLC patients as well as that according to pathological subtypes (ADC, SCC, LCC, ASC and others). Kaplan–Meier analysis of overall survival (F) and relapse-free survival (G) of patients with NSCLC (P = 0.002 by log-rank test) according to expression of Wnt2 protein. Vertical bars on survival curves indicate censored cases.

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Expression of Wnt2 in normal tissue and non-small cell lung cancers. (A) expression of Wnt2 in 8 representative pairs of NSCLC lung cancer tissues (T) and adjacent normal lung (N) tissues detected by RT-PCR (top) and Western blot (bottom). The results were processed with ImageJ (B). (C) immunohistochemical staining of Wnt2 protein in 5 representative pairs of histologic types of NSCLC (ADC, BAC, ASC, SCC and LCC) and adjacent normal lung tissues. (D) examples are shown for absent, low, median and high Wnt2 expression in NSCLC cancer tissues. (E) distribution of Wnt2 immunohistochemical results in 264 NSCLC patients as well as that according to pathological subtypes (ADC, SCC, LCC, ASC and others). Kaplan–Meier analysis of overall survival (F) and relapse-free survival (G) of patients with NSCLC (P = 0.002 by log-rank test) according to expression of Wnt2 protein. Vertical bars on survival curves indicate censored cases.

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Immunohistochemical staining, Staining

Association between WNT2 IHC staining and patients’ clinicopathological parameters (n = 264)

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Association between WNT2 IHC staining and patients’ clinicopathological parameters (n = 264)

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Immunohistochemistry, Expressing

Cox’s proportional hazards model analysis of prognostic factors in patients with NSCLCs

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Cox’s proportional hazards model analysis of prognostic factors in patients with NSCLCs

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques:

Expression of Wnt2 expression in 5 NSCLC cell lines. Expression of Wnt2 in 5 NSCLC cell lines (3 adenocarcinoma, A549, H1299 and H1975; 1 large cell cancer, H460; 1 squamous cell cancer, SK-MES-1) was detected by RT-PCR (A) and Western blot (B) and then analyzed with ImageJ (C). (D) The Wnt2 protein in the supernatant of the 5 aforementioned cell lines was detected by ELISA assay.

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Expression of Wnt2 expression in 5 NSCLC cell lines. Expression of Wnt2 in 5 NSCLC cell lines (3 adenocarcinoma, A549, H1299 and H1975; 1 large cell cancer, H460; 1 squamous cell cancer, SK-MES-1) was detected by RT-PCR (A) and Western blot (B) and then analyzed with ImageJ (C). (D) The Wnt2 protein in the supernatant of the 5 aforementioned cell lines was detected by ELISA assay.

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay

Serologic level of Wnt2 determined by ELISA experiments in patients with NSCLC and healthy volunteers. A: Serum Wnt2 levels of patients with NSCLC (patients were divided into ADC and non-ADC groups) and healthy controls. Black line indicates the cutoff value (4.39 ng/ml) which was determined by ROC curve in MedCalc programme. B: Distribution of Wnt2 IHC staining results of 67 NSCLC patients according to serum Wnt2 level. C: Serum levels of Wnt2 before and after radical resection of primary tumors in 12 NSCLC patients (P = 0.001). Black lines indicate cutoff values. D: ROC curve analysis of serum Wnt2 (Red line) and CEA (green line) as serum markers for adenocarcinoma of lung cancer. x-axis: 1-Specificity; y-axis, sensitivity. *: P < 0.05.

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Serologic level of Wnt2 determined by ELISA experiments in patients with NSCLC and healthy volunteers. A: Serum Wnt2 levels of patients with NSCLC (patients were divided into ADC and non-ADC groups) and healthy controls. Black line indicates the cutoff value (4.39 ng/ml) which was determined by ROC curve in MedCalc programme. B: Distribution of Wnt2 IHC staining results of 67 NSCLC patients according to serum Wnt2 level. C: Serum levels of Wnt2 before and after radical resection of primary tumors in 12 NSCLC patients (P = 0.001). Black lines indicate cutoff values. D: ROC curve analysis of serum Wnt2 (Red line) and CEA (green line) as serum markers for adenocarcinoma of lung cancer. x-axis: 1-Specificity; y-axis, sensitivity. *: P < 0.05.

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry

The correlation between immunohistochemical results of Wnt2 and corresponding serum Wnt2 level in 67 patients with NSCLC

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: The correlation between immunohistochemical results of Wnt2 and corresponding serum Wnt2 level in 67 patients with NSCLC

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Immunohistochemical staining, Staining

Enhanced or inhibited growth of lung cancer cells by stably overexpressing exogenous Wnt2 or siRNAs against WNT2. (A) H460 cells were stably transfected with pLV-WNT2 vector or empty vector as control via lentivirus. Western blot assays were used to verify the transfection efficiency. The growth rate of the cells was evaluated by the EDU incorporation assays (B) and colony formation assays (C). (D) Gene knockdown effect on WNT2 expression in H1299 cells by siRNAs against WNT2 and scramble siRNAs. Forty-eight hours after transient transfection, the growth rate of the cells was evaluated by the EDU incorporation assays (E) and colony formation assays (F). *: P < 0.05.

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Enhanced or inhibited growth of lung cancer cells by stably overexpressing exogenous Wnt2 or siRNAs against WNT2. (A) H460 cells were stably transfected with pLV-WNT2 vector or empty vector as control via lentivirus. Western blot assays were used to verify the transfection efficiency. The growth rate of the cells was evaluated by the EDU incorporation assays (B) and colony formation assays (C). (D) Gene knockdown effect on WNT2 expression in H1299 cells by siRNAs against WNT2 and scramble siRNAs. Forty-eight hours after transient transfection, the growth rate of the cells was evaluated by the EDU incorporation assays (E) and colony formation assays (F). *: P < 0.05.

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Stable Transfection, Transfection, Plasmid Preparation, Control, Western Blot, Knockdown, Expressing

The correlation between Wnt2 and Ki67 expression in tumor tissues of NSCLC. Representative immunohistological images of Wnt2 and Ki67 correlative expression in consecutive sections from human NSCLC surgical specimens. Original magnification, 200 ×; scale bars, 100 mm.

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: The correlation between Wnt2 and Ki67 expression in tumor tissues of NSCLC. Representative immunohistological images of Wnt2 and Ki67 correlative expression in consecutive sections from human NSCLC surgical specimens. Original magnification, 200 ×; scale bars, 100 mm.

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Expressing

Statistical analysis of immunohistochemical results of Wnt2 and Ki67 labelling index in 264 human NSCLC surgical samples

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Statistical analysis of immunohistochemical results of Wnt2 and Ki67 labelling index in 264 human NSCLC surgical samples

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Immunohistochemical staining

Overexpression of Wnt2 in the tumor microenvironment enhances tumor progression in vivo. A total of 5 × 106 pLV-Wnt2 or pLV-vector cells was injected s.c. into each mouse (A). The tumor size was measured every week (B); at the end of the experiments, tumors were resected from sacrificed mice and photographed (C). (D) pLV-Wnt2 or pLV-vector tumors from mice were analyzed for the expression of Wnt2 and Ki67 by immunofluorescent assay and microscopy. Original magnification, 200 ×; scale bars, 100 um. (E) Five random fields from each tumor were analyzed and mean Ki67 positive cell counts for each tumor were analyzed by Student t test. *: P < 0.05.

Journal: American Journal of Cancer Research

Article Title: Wnt2 promotes non-small cell lung cancer progression by activating WNT/β-catenin pathway

doi:

Figure Lengend Snippet: Overexpression of Wnt2 in the tumor microenvironment enhances tumor progression in vivo. A total of 5 × 106 pLV-Wnt2 or pLV-vector cells was injected s.c. into each mouse (A). The tumor size was measured every week (B); at the end of the experiments, tumors were resected from sacrificed mice and photographed (C). (D) pLV-Wnt2 or pLV-vector tumors from mice were analyzed for the expression of Wnt2 and Ki67 by immunofluorescent assay and microscopy. Original magnification, 200 ×; scale bars, 100 um. (E) Five random fields from each tumor were analyzed and mean Ki67 positive cell counts for each tumor were analyzed by Student t test. *: P < 0.05.

Article Snippet: A human Wnt2 ELISA kit (CUSABIO) was used to detect Wnt2 concentration in the serum samples or the supernatants of cell cultures.

Techniques: Over Expression, In Vivo, Plasmid Preparation, Injection, Expressing, Microscopy

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